Native Proteins and DNAs

Topoisomerase II alpha | Topoisomerase II alpha Assay Kit | FACT | SAE1/SAE2 |
In Vitro Sumoylation Kit | HeLa Core Histones | In Vitro Chromatin Assembly Kit | HnRNP U | Nucleophosmin/B23 | Nucleolin | Ku70/86 | HSP90 | Nucleolin EMSA Kit | Nucleophosmin EMSA Kit | pNAS | Kinetoplast DNA | Anti-RNA helicase A Ab | Anti-Cyclophilin B Ab


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Human topoisomerase II alpha
  • Cat No: T-001
  • Price: $ 249
  • Size: 200 units*
  • Purity: >95%
  • Intactness: >95%
  • Stability: No detectable change in activity up to 12 months of storage at -70oC and after 20 cycles of freeze-thawing.
Key features: Highly active protein purified from HeLa cells. Specific activity is approximately 500K units per mg protein. *1 unit is defined as the activity to catalyze decatenation of 250 ng of subtrate DNA in 15 min at 37oC. In addition to highly active human topoisomerase II alpha, the following reagents will be provided.
  • 10x Reaction Buffer (250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, 0.1 M  MgCl2, and 20 mM ATP.
  • 10x Control Reaction Buffer (250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, and 0.1 M  MgCl2.
  • 10x BSA (2 mg/ml, 250 ul).



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Human topoisomerase II alpha
  • Cat No: T-002
  • Price: $ 999
  • Size: 1000 units*
  • Purity: >95%
  • Intactness: >95%
  • Stability: No detectable change in activity up to 12 months of storage at -70oC and after 20 cycles of freeze-thawing.
Key features: Highly active protein purified from HeLa cells. Specific activity is approximately 500K units per mg protein. *1 unit is defined as the activity to catalyze decatenation of 250 ng of catenated DNA in 15 min at 37oC. In addition to highly active human topoisomerase II alpha, the following reagents will be provided.
  • 10x Reaction Buffer (1250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, 0.1 M  MgCl2, and 20 mM ATP.
  • 10x Control Reaction Buffer (1250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, and 0.1 M  MgCl2.
  • 10x BSA (2 mg/ml, 1250 ul)


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Topoisomerase II alpha assay Kit
  • Cat No: TA-001
  • Price: $ 349
  • Size: 100 reactions
Key features: In addition to highly active human topoisomerase II alpha, this assay kit contains the following reagents.
  • 10x Reaction Buffer (250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, 0.1 M  MgCl2, and 20 mM ATP.
  • 10x Control Reaction Buffer (250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, and 0.1 M  MgCl2.
  • 10x BSA (2 mg/ml, 250 ul)
  • Topoisomerase II alpha (100 units)
  • Catenated kinetoplast DNA (25 ug)
  • 6% SDS (250 ul)
  • 3 mg/ml Proteinase-K (250 ul)
  • 5x Loading buffer (500 ul)
For detailed info, download topo-manual.


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Human FACT
  • Cat No: F-001
  • Price: $ 249
  • Size: 2 ug
  • Purity: >95%
  • Intactness: >95%
Key features: Human native heterodimeric complex purified from HeLa cells.


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Human FACT
  • Cat No: F-002
  • Price: $ 999
  • Size: 10 ug
  • Purity: >95%
  • Intactness: >95%
Key features: Human native heterodimeric complex purified from HeLa cells.


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Human SAE1/SAE2
  • Cat No: S-001
  • Price: $ 349
  • Size: 100 units*
  • Purity: >95%
  • Intactness: >95%
Key features: Highly active heterodimeric protein purified from HeLa cells. *1 unit is defined as the activity to catalyze sumoylation of 400 pmol of substrate peptide in 120 min at 37oC.


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In vitro Sumoylation Kit
  • Cat No: SA-001
  • Price: $ 499
  • Size: 50 reactions
Key features: In addition to highly active human SAE1/SAE2 (50 units), this assay kit contains the following reagents.
  • 10x Reaction Buffer (200 ul): 0.2 M Hepes-NaOH, pH 7.4, 50 mM MgCl2, 20 mM ATP, 0.5 mM PMSF, and 1 mg/ml BSA.
  • SUMO-1 (50 ul, 1 mg/ml)
  • GST-UBC9 (50 ul, 0.4 mg/ml)
  • Control peptide (Gst-IkBa, 50 ul, 0.5 mg/ml)
  • 5x SDS sample buffer (250 ul)
For detailed info, download sumo-manual.


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In vitro Sumoylation Kit
  • Cat No: SB-001
  • Price: $ 499
  • Size: 50 reactions
Key features: In addition to highly active human SAE1/SAE2 (50 units), this assay kit contains the following reagents.
  • 10x Reaction Buffer (200 ul): 0.2 M Hepes-NaOH, pH 7.4, 50 mM MgCl2, 20 mM ATP, 0.5 mM PMSF, and 1 mg/ml BSA.
  • SUMO-1 (50 ul, 1 mg/ml)
  • GST-UBC9 (50 ul, 0.4 mg/ml)
  • Control peptide (Flag/Gst-RHA137, 50 ul, 0.5 mg/ml)
  • 5x SDS sample buffer (250 ul)
For detailed info, download sumo-manual.


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HeLa core histones
  • Cat No: H-001
  • Price: $ 249
  • Size: 100 ug
  • Purity: >95%
  • Intactness: >95%
Key features: Isolated from HeLa nuclei. Highly active in assays such as histone acetylation and chromatin assembly. In addition to core histones, BSA (100 ug, 2 mg/ml) will be provided as a control protein.


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In vitro Chromatin Assembly Kit
  • Cat No: HA-001
  • Price: $ 499
  • Size: In vitro chromatinization of up to 100 ug of DNA.
  • Efficiency: >95%
Key features: The use of an internal control DNA makes it possible to monitor the efficiency of in vitro chromatin assembly with target DNA of either linear or circular conformation. In vitro chromatin assembly kit contains the following reagents:
  • 10x Reaction Buffer (200 ul): 0.2 M Hepes-NaOH, pH 7.4, 0.1 mM EDTA, 0.5 mM PMSF, and 10 mM DTT.
  • Control DNA template (pNAS, 2 ug, 0.1 mg/ml)
  • PCR primer mix (40 ul, 100 pmol/ul)
  • 5 M NaCl (200 ul)
  • HeLa core histones (50 ul, 2 mg/ml)
  • Dilution Buffer (0.5 ml): 20 mM Hepes-NaOH, pH 7.4, 0.1 mM EDTA, 0.5 mM PMSF, and 1 mM DTT
  • Storage Buffer (1 ml): 40 mM Hepes-NaOH, pH 7.4, 0.2 mM EDTA, 0.5 mM PMSF, 2 mM DTT, 0.1% NP-40, 0.6 mg/ml BSA, and 30% glycerol.
For detailed info, download cak-manual.



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HnRNP U
(Heterogeneous ribonucleoprotein U)

  • Cat No: U-001
  • Price: $ 249
  • Size: 5 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%).



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HnRNP U
(Heterogeneous ribonucleoprotein U)
  • Cat No: U-002
  • Price: $ 999
  • Size: 25 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%).



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NPM (Nucleophosmin/B23)
  • Cat No: B-001
  • Price: $ 249
  • Size: 2 ug
  • Purity: >95%
Key features: NPM is a native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%). Purified NPM is in a multimer conformation and highly active in interacting with RNA.



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NPM (Nucleophosmin/B23)
  • Cat No: B-002
  • Price: $ 999
  • Size: 10 ug
  • Purity: >95%
Key features: NPM is a native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%). Purified NPM is in a multimer conformation and highly active in interacting with RNA.



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Nucleolin
  • Cat No: NN-001
  • Price: $ 299
  • Size: 2 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%). Nucleolin harbors an activity to efficiently interact with RNA in vitro, without changing the overall integrity of substrate RNA.



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Nucleolin
  • Cat No: NN-002
  • Price: $ 1199
  • Size: 10 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%). Nucleolin harbors an activity to efficiently interact with RNA in vitro, without changing the overall integrity of substrate RNA.



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Ku70/86
  • Cat No: KU-001
  • Price: $ 349
  • Size: 2 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%).



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Ku70/86
  • Cat No: KU-002
  • Price: $ 1299
  • Size: 10 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%)



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Nucleolin EMSA Kit
  • Cat No: NS-001
  • Price: $ 649
  • Size: 100 reactions
Key features:
Nucleolin, isolated from HeLa cells, harbors an activity to efficiently interact with RNA in vitro, without changing the overall integrity of substrate RNA. In a standard reaction (10 ul), nucleolin (50 ng) forms complexes with virtually all RNA substrates (1 nM) provided. However, the optimal concentration of nucleolin should be emperically determined for each RNA substrate of researcher's interest. The following reagents will be provided:
  • Nucleolin: 5 ug (0.1 mg/ml).
  • 10x Reaction buffer (0.25 ml): 0.2 M Hepes-NaOH, pH 7.4, 1 M NaCl, 10 mM DTT, and 2 mg/ml BSA.
  • 5x Loading buffer (0.5 ml); 0.1 M Tris-HCl, pH 7.4, 5 mM EDTA, 0.05% NP-40, 0.01% bromophenol blue, 0.01% xylene cyanol, and 50% glycerol
  • Protein dilution buffer (0.5 ml): 20 mM Hepes-NaOH, pH 7.4, 50 mM NaCl, 0.1 mM EDTA, 0.5 mM PMSF, 0.2 mg/ml bovine serum albumin, 0.05% Noniodit P-40, a cocktail of protease inhibitors, and 20% glycerol.
  • Instruction manual.



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Nucleophosmin EMSA Kit
  • Cat No: BS-001
  • Price: $ 549
  • Size: 100 reactions
  • Storage: -70oC
Key features: Purified NPM is in a multimer conformation and highly active in interacting with RNA. In a standard reaction (10 ul), NPM (50 ng) forms stable complexes with virtually all RNA substrates (1 nM) provided. However, the optimal concentration of NPM1 should be emperically determined for each RNA substrate of researcher's interest. The following reagents will be provided:
  • NPM: 5 ug (0.1 mg/ml).
  • 10x Reaction Buffer (0.25 ml): 0.2 M Hepes-NaOH, pH 7.4, 1 M NaCl, 10 mM DTT, and 2 mg/ml BSA.
  • 5x Loading buffer (0.5 ml): 0.1 M Tris-HCl, pH 7.4, 5 mM EDTA, 0.05% NP-40, 0.01% bromophenol blue, 0.01% xylene cyanol, and 50% glycerol
  • Protein dilution buffer (0.5 ml): 20 mM Hepes-NaOH, pH 7.4, 50 mM NaCl, 0.1 mM EDTA, 0.5 mM PMSF, 0.2 mg/ml bovine serum albumin, 0.05% Noniodit P-40, a cocktail of protease inhibitors, and 20% glycerol.
  • Instruction manual.





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Hsp90
  • Cat No: HP-001
  • Price: $ 249
  • Size: 10 ug
  • Purity: >95%
Key features: Hsp90b is a molecular chaperone regulating the function of client proteins, including signalling proteins. Hsp90b is isolated from nuclear extracts of HeLa cells through ammonium sulfate fractionation, ion-exchage column chromatographic steps utilizing Q-Sepharose, S-Sepharose, hydroxyapatite, and phosphocellulose, followed by ultracentrifugal separation on a glycerol gradient (20 to 40%). Hsp90b is highly pure and is in a native homodimeric conformation.





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Hsp90
  • Cat No: HP-002
  • Price: $ 999
  • Size: 50 ug
  • Purity: >95%
Key features: Hsp90b is a molecular chaperone regulating the fuction of client proteins, including signalling proteins. Hsp90b is isolated from nuclear extracts of HeLa cells through ammonium sulfate fractionation, ion-exchage column chromatographic steps utilizing Q-Sepharose, S-Sepharose, hydroxyapatite, and phosphocellulose, followed by ultracentrifugal separation on a glycerol gradient (20 to 40%). Hsp90b is highly pure and is in a native homodimeric conformation.




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pNAS
  • Cat No: P-001
  • Price: $ 249
  • Size: 25 ug
  • Storage: -70oC
  • Stability: No nicking or cleavage after 10 cycles of freeze-thawing.
Key features: A 3-kb plasmid DNA containing X. laevis 5S ribosomal DNA (~130 bp) that promotes the nucleosome assembly.



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Kinetoplast DNA
  • Cat No: K-001
  • Price: $ 199
  • Size: 25 ug
  • Storage: -70oC
  • Stability: No nicking or cleavage after 10 cycles of freeze-thawing.
Key features: Highly catenated kinetoplast DNA, an ideal substrate to measure the ATP-dependent decatenation reaction catalyzed by topoisomerase II alpha. In addition to kinetoplast DNA, the following reagents will be provided.
  • 10x Reaction Buffer (250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, 0.1 M  MgCl2, and 20 mM ATP
  • 10x BSA (2 mg/ml, 250 ul)



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Kinetoplast DNA
  • Cat No: K-002
  • Price: $ 799
  • Size: 250 ug
  • Storage: -70oC
  • Stability: No nicking or cleavage after 10 cycles of freeze-thawing.
Key features: Highly catenated kinetoplast DNA, an ideal substrate to measure the ATP-dependent decatenation reaction catalyzed by topoisomerase II alpha. In addition to kinetoplast DNA, the following reagents will be provided.
  • 10x Reaction Buffer (1250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, 0.1 M  MgCl2, and 20 mM ATP
  • 10x BSA (2 mg/ml, 1250 ul)
 



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Anti-RNA helicase A Abs
  • Cat No: PA-001
  • Price: $ 249
  • Size: 0.1 ml
  • Storage: 4oC
  • Application: WB/IP
  • Specificity: humans, mice, rat, cows
Key features: Rabbit polyclonal antibodies raised against full-length RHA. For WB, use 1:10,000 dilution for the detection of RNA helicase A from whole cell extract (WCE) or nuclear extract equivalent to 20 ug of protein. Since it is raised against full-length native protein, antibodies are capable of detecting not only intact RHA but also processed form of RHA, as shown in the figure. Higher molecular weight of murine RHA is due to the presence of much longer C-terminal GGYGG domain.



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Anti-RNA helicase A Abs
  • Cat No: PA-002
  • Price: $ 999
  • Size: 0.5 ml
  • Storage: 4oC
  • Application: WB/IP
  • Specificity: humans, mice, rat, cows
Key features: Rabbit polyclonal antibodies raised against full-length RHA. For WB, use 1:10,000 dilution
for the detection of RNA helicase A from whole cell extract (WCE) or nuclear extract equivalent to 20 ug of protein. Since it is raised against full-length native protein, antibodies are capable of detecting not only intact RHA but also processed form of RHA, as shown in the figure. Higher molecular weight of murine RHA is due to the presence of much longer C-terminal GGYGG domain.




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Anti-RNA helicase A Abs
  • Cat No: PA-003
  • Price: $ 1499
  • Size: 0.5 mlx 2
  • Storage: 4oC
  • Application: WB/IP
  • Specificity: humans, mice, rat, cows
Key features: Rabbit polyclonal antibodies raised against full-length RHA. For WB, use 1:10,000 dilution
for the detection of RNA helicase A from whole cell extract (WCE) or nuclear extract equivalent to 20 ug of protein. Since it is raised against full-length native protein, antibodies are capable of detecting not only intact RHA but also processed form of RHA, as shown in the figure. Higher molecular weight of murine RHA is due to the presence of much longer C-terminal GGYGG domain.




Anti-Cyclophilin B Abs
  • Cat No: PC-001
  • Price: $ 249
  • Size: 0.1 ml
  • Storage: 4oC
  • Application: WB
  • Specificity: humans, mice, rat, cows
Key features: Rabbit polyclonal antibodies raised against full-length cyclophilin B. For WB, use 1:5,000 dilution
for the detection of cyclophilin B from whole cell extract (WCE) or nuclear extract equivalent to 20 ug of protein.



Anti-Cyclophilin B Abs
  • Cat No: PC-002
  • Price: $ 999
  • Size: 0.5 ml
  • Storage: 4oC
  • Application: WB
  • Specificity: humans, mice, rat, cows
Key features: Rabbit polyclonal antibodies raised against full-length cyclophilin B. For WB, use 1:5,000 dilution
for the detection of cyclophilin B from whole cell extract (WCE) or nuclear extract equivalent to 20 ug of protein.



Anti-Cyclophilin B Abs
  • Cat No: PC-003
  • Price: $ 1499
  • Size: 0.5 mlx 2
  • Storage: 4oC
  • Application: WB
  • Specificity: humans, mice, rat, cows
Key features: Rabbit polyclonal antibodies raised against full-length cyclophilin B. For WB, use 1:5,000 dilution
for the detection of cyclophilin B from whole cell extract (WCE) or nuclear extract equivalent to 20 ug of protein.


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